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The Effect of HEXIM1 Knockdown on Gene Expression and Its Biological Function in Undifferentiated Keratinocytes

Author(s)

Xinmiao Wu

Affiliation(s)

School of Science , Xi’an Jiaotong-Liverpool University, Suzhou, China

Abstract

HEXIM1 is a multifunctional protein that plays significant roles in various biological processes, including transcriptional regulation, cell cycle, and stress responses. It primarily functions by binding to the 7SK small nuclear ribonucleoprotein (7SK snRNP), thereby inhibiting the activity of the P-TEFb complex to regulate gene transcription. In the context of skin self-renewal and repair, the accurate differentiation of epidermal progenitor cells (EPCs) is essential. This study aims to elucidate the specific function and molecular mechanisms of HEXIM1 protein in this process through transcriptome data analysis. The results indicate that HEXIM1 knockdown leads to alterations in the gene expression profiles of undifferentiated keratinocytes. Furthermore, genes with upregulated expression are predominantly enriched in pathways related to "epidermal development" and "keratinization," suggesting that HEXIM1 can suppress the expression of differentiation-related genes, thus maintaining the progenitor cell state.

Keywords

HEXIM1; Transcriptome Analysis; Cell Differentiation

References

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